| Host: |
Rabbit |
| Applications: |
IHC/WB |
| Reactivity: |
Mouse/Rat/Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NBR1 (290-340 aa) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB (confirmed by recombinant protein). A dilution of 1: 300 to 1: 2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
Nbr1 |
| Gene ID: |
17966 |
| Uniprot ID: |
NBR1_MOUSE |
| Immunogen Region: |
290-340 aa |
| Specificity: |
Specific for NBR1 |
| Immunogen: |
A synthetic peptide from aa region 290-340 of mouse NBR1 conjugated to an immunogenic carrier protein was used as the antigen. The peptide is homologous in rat and shares 98% identity with human sequence. |
| Post Translational Modifications | Phosphorylated by GSK3A.this phosphorylation inhibits NBR1 involvement in the formation of ubiquitinated protein aggregates. |
| Function | Ubiquitin-binding autophagy adapter that participates in different processes including host defense or intracellular homeostasis. Possesses a double function during the selective autophagy by acting as a shuttle bringing ubiquitinated proteins to autophagosomes and also by participating in the formation of protein aggregates. Plays a role in the regulation of the innate immune response by modulating type I interferon production and targeting ubiquitinated IRF3 for autophagic degradation. In response to oxidative stress, promotes an increase in SQSTM1 levels, phosphorylation, and body formation by preventing its autophagic degradation. In turn, activates the KEAP1-NRF2/NFE2L2 antioxidant pathway. Plays also non-autophagy role by mediating the shuttle of IL-12 to late endosome for subsequent secretion. |
| Protein Name | Next To Brca1 Gene 1 ProteinMembrane Component Chromosome 17 Surface Marker 2 HomologNeighbor Of Brca1 Gene 1 Protein |
| Database Links | Reactome: R-MMU-9664873 |
| Cellular Localisation | CytoplasmCytoplasmic VesicleAutophagosomeLysosomeMyofibrilSarcomereM LineIn Cardiac Muscles Localizes To The Sarcomeric M LineIs Targeted To Lysosomes For Degradation |
| Alternative Antibody Names | Anti-Next To Brca1 Gene 1 Protein antibodyAnti-Membrane Component Chromosome 17 Surface Marker 2 Homolog antibodyAnti-Neighbor Of Brca1 Gene 1 Protein antibodyAnti-Nbr1 antibodyAnti-M17s2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
