• Immunohistochemistry analysis of paraffin-embedded mouse spleen using NASP Antibody (STJ29018) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human brain astrocytoma using NASP Antibody (STJ29018) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using NASP Antibody (STJ29018) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NASP antibody (STJ29018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NASP antibody (180-449) (STJ29018)

SKU:
STJ29018

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NASP
Gene ID: 4678
Uniprot ID: NASP_HUMAN
Immunogen Region: 180-449
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 180-449 of human NASP (NP_689511.2).
Immunogen Sequence: WDMLDLAKIIFKRQETKEAQ LYAAQAHLKLGEVSVESENY VQAVEEFQSCLNLQEQYLEA HDRLLAETHYQLGLAYGYNS QYDEAVAQFSKSIEVIENRM AVLNEQVKEAEGSSAEYKKE IEELKELLPEIREKIEDAKE SQRSGNVAELALKATLVESS TSGFTPGGGGSSVSMIASRK PTDGASSSNCVTDISHLVRK KRKPEEESPRKDDAKKAKQE PEVNGGSGDAVPSGNEVSE
Tissue Specificity Isoform 1 is testis- and sperm-specific.
Function Component of the histone chaperone network. Binds and stabilizes histone H3-H4 not bound to chromatin to maintain a soluble reservoir and modulate degradation by chaperone-mediated autophagy. Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA. Isoform 1: Stabilizes soluble histone H3-H4. Isoform 2: Stabilizes soluble histone H3-H4.
Protein Name Nuclear Autoantigenic Sperm Protein
Nasp
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Nuclear Autoantigenic Sperm Protein antibody
Anti-Nasp antibody
Anti-NASP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance