Anti-NANOG antibody (160-305) (STJ11104720)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal NANOG (160-305) antibody for use in WB and ELISA in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Nanog |
| Gene ID | 71950 |
| Uniprot ID | NANOG_MOUSE |
| Immunogen Region | 160-305 |
| Immunogen Sequence | LKTSNGLIQKGSAPVEYPSI HCSYPQGYLVNASGSLSMWG SQTWTNPTWSSQTWTNPTWN NQTWTNPTWSSQAWTAQSWN GQPWNAAPLHNFGEDFLQPY VQLQQNFSASDLEVNLEATR ESHAHFSTPQALELFLNYSV TPPGEI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 160-305 of mouse NANOG (NP_082292.1). |
Additional Info
| Tissue Specificity | Not expressed in oocytes and spermatogonia (at protein level). Not expressed in many somatic organs, ovary, testis, fibroblast and hematopoietic cell lines. |
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAATGTGT-3' or 5'-CGGACGCGCATTANGC-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| Protein Name | Homeobox Protein NanogEs Cell-Associated Protein 4Early Embryo Specific Expression Nk-Type Homeobox ProteinHomeobox Transcription Factor Nanog |
| Database Links | |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Nanog antibodyAnti-Es Cell-Associated Protein 4 antibodyAnti-Early Embryo Specific Expression Nk-Type Homeobox Protein antibodyAnti-Homeobox Transcription Factor Nanog antibodyAnti-Nanog antibodyAnti-Ecat4 antibodyAnti-Enk antibody |
Information sourced from Uniprot.org