| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-NANOG (160-305) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
Nanog |
| Gene ID: |
71950 |
| Uniprot ID: |
NANOG_MOUSE |
| Immunogen Region: |
160-305 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 160-305 of mouse NANOG (NP_082292.1). |
| Immunogen Sequence: |
LKTSNGLIQKGSAPVEYPSI HCSYPQGYLVNASGSLSMWG SQTWTNPTWSSQTWTNPTWN NQTWTNPTWSSQAWTAQSWN GQPWNAAPLHNFGEDFLQPY VQLQQNFSASDLEVNLEATR ESHAHFSTPQALELFLNYSV TPPGEI |
| Tissue Specificity | Not expressed in oocytes and spermatogonia (at protein level). Not expressed in many somatic organs, ovary, testis, fibroblast and hematopoietic cell lines. |
| Function | Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. Acts as a transcriptional activator or repressor. Binds optimally to the DNA consensus sequence 5'-TAATGTGT-3' or 5'-CGGACGCGCATTANGC-3'. Binds to the POU5F1/OCT4 promoter. Able to autorepress its expression in differentiating (ES) cells: binds to its own promoter following interaction with ZNF281/ZFP281, leading to recruitment of the NuRD complex and subsequent repression of expression. When overexpressed, promotes cells to enter into S phase and proliferation. |
| Protein Name | Homeobox Protein NanogEs Cell-Associated Protein 4Early Embryo Specific Expression Nk-Type Homeobox ProteinHomeobox Transcription Factor Nanog |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Homeobox Protein Nanog antibodyAnti-Es Cell-Associated Protein 4 antibodyAnti-Early Embryo Specific Expression Nk-Type Homeobox Protein antibodyAnti-Homeobox Transcription Factor Nanog antibodyAnti-Nanog antibodyAnti-Ecat4 antibodyAnti-Enk antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
