Host: | Goat |
Applications: | Pep-ELISA/WB |
Reactivity: | Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Goat polyclonal antibody anti-Nalp12/Nlrp12-Rat (Internal) is suitable for use in ELISA and Western Blot research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 100kDa band observed in Mouse Liver and Spleen lysates (calculated MW of 119kDa according to NP_001028603.1). The observed molecular weight corresponds to earlier findings in l |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Immunogen Region: | Internal |
Accession Number: | NP_001028603.1 |
Immunogen Sequence: | KPGTPTFKVPANQR |
Function | Plays an essential role as an potent mitigator of inflammation. Primarily expressed in dendritic cells and macrophages, inhibits both canonical and non-canonical NF-kappa-B and ERK activation pathways. Functions as a negative regulator of NOD2 by targeting it to degradation via the proteasome pathway. In turn, promotes bacterial tolerance. Inhibits also the DDX58-mediated immune signaling against RNA viruses by reducing the E3 ubiquitin ligase TRIM25-mediated 'Lys-63'-linked DDX58 activation but enhancing the E3 ubiquitin ligase RNF125-mediated 'Lys-48'-linked DDX58 degradation. Acts also as a negative regulator of inflammatory response to mitigate obesity and obesity-associated diseases in adipose tissue. |
Protein Name | Nacht - Lrr And Pyd Domains-Containing Protein 12Monarch-1Pyrin-Containing Apaf1-Like Protein 7Pypaf7 |
Cellular Localisation | Cytoplasm |
Alternative Antibody Names | Anti-Nacht - Lrr And Pyd Domains-Containing Protein 12 antibodyAnti-Monarch-1 antibodyAnti-Pyrin-Containing Apaf1-Like Protein 7 antibodyAnti-Pypaf7 antibodyAnti-Nlrp12 antibodyAnti-NALP12 antibody |
Information sourced from Uniprot.org