• STJ73617 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73617 Flow cytometric analysis of paraformaldehyde fixed NIH3T3 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-Nalp10/Nlrp10-Mouse antibody (N-Term) (STJ73617)

SKU:
STJ73617-100

£312.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Nalp10/Nlrp10-Mouse (N-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
IF: Strong expression of the protein seen in the cytoplasm of NIH3T3 cells. Recommended concentration: 10µg/ml.

FC:Flow cytometric analysis of NIH3T3 cells. Recommended concentration: 1
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Immunogen Region: N-Term
Accession Number: NP_780741.1
Immunogen Sequence: NDLEENSFKTLKFH
Function Inhibits autoprocessing of CASP1, CASP1-dependent IL1B secretion, PYCARD aggregation and PYCARD-mediated apoptosis but not apoptosis induced by FAS or BID. Displays anti-inflammatory activity. Required for immunity against C.albicans infection. Involved in the innate immune response by contributing to proinflammatory cytokine release in response to invasive bacterial infection. Contributes to T-cell-mediated inflammatory responses in the skin. Plays a role in protection against periodontitis through its involvement in induction of IL1A via ERK activation in oral epithelial cells infected with periodontal pathogens. Exhibits both ATPase and GTPase activities.
Protein Name Nacht - Lrr And Pyd Domains-Containing Protein 10
Cellular Localisation Cytoplasm
Cell Membrane
Peripheral Membrane Protein
Cytoplasmic Protein Which Is Recruited To The Cell Membrane By Nod1 Following Invasive Bacterial Infection
Alternative Antibody Names Anti-Nacht - Lrr And Pyd Domains-Containing Protein 10 antibody
Anti-Nlrp10 antibody
Anti-Nalp10 antibody
Anti-Pynod antibody

Information sourced from Uniprot.org

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