• Immunoprecipitation analysis of 25 Mu g total RNA extracts of Arabidopsis (25 Mu g total RNA extracts of Arabidopsis was diluted to 200 µl IP buffer system) using 2ul m6A antibody (STJ11103883).
  • U2OS cells pre-treated with BrdU were subjected UVC irradiation incubated at 37 °C for the indicated time, and stained for m6A Polyclonal Antibody (STJ11103883). DAPI, 4′, 6-diamidino-2-phenylindole. Global UVC irradiation exceed cytoplasmic leavel, peaking at 2 min after irradiation and diminishing over the following 8 min.
  • The m6A rabbit polyclonal antibody (STJ11103883) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine. Oligomer 8- ATAACTGG-m6A-CCGAATGGOligomer 9- ATAACTGGACCGAATGGOligomer 10- AAAAAAAAAAAAAAAA-biotin.
  • The m6A rabbit polyclonal antibody (500ng/100ng, STJ11103883) are tested in Dot Blot against total RNA of Arabidopsis. (This image is courtesy of an anonymous Abreview)
  • The m6A rabbit polyclonal antibody (4µg, STJ11103883) are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine (100pmol for each oligomer). Oligomer 1- N6-methyladenosine (m6A-CUGGUAACGAAUGGCU-G-Biotin) Oligomer 2- unmodified adenosineOligomer 3- unmodified adenosine (AAAAAAAAAAAAAAAAA-Biotin)

Anti-N6-methyladenosine/m6A antibody (STJ11103883)

SKU:
STJ11103883

£129.50 - £639.50
Current Stock:
Host: Rabbit
Applications: IF
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-N6-methyladenosine/m6A is suitable for use in Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: DB 1:500-1:2000
IF/ICC 1:50-1:200meRIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Chemical compounds corresponding to N6-methyladenosine/m6A.
Background Discovered in the 1970s, m6A is the most prevalent internal modification in polyadenylated mRNAs and long non-coding RNAs (lncRNAs) in higher eukaryotes. m6A is widely conserved among eukaryotic species that range from yeast, plants, flies to mammals, as well as among viral RNAs with a nuclear phase. The m6A-based modification is associated with a well-defined RNA motif, RRACH (R: A/G, H: A/C/U). As a representative of the epitranscriptome, m6A mRNA modifications participate in many vital activities in the cell, including stem cell self-renewal and differentiation, mRNA transcription, alternative splicing, nuclear export, translation, degradation, and microRNA processing. These processes determine the expression or inactivation of specific genes, which is vital for growth and development. (PMID: 30416848; PMID: 24662220; PMID: 30429466)

Information sourced from Uniprot.org

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