• RNA Immunoprecipitation was performed on 100 µg mouse liver total RNA, using 5 µg of the N6-methyladenosine/ m6A Rabbit mAb. An equal amount of IgG was used as negative control. The immunoprecipitated RNA was verified by using HS6ST1 as PCR primer of qRT-PCR. The picture shows the relative enrichment multiple of HS6ST1 site.
  • Immunofluorescence analysis of U-2 OS treated with UV (30s) +Brdu (0min) and U-2 OS treated with UV (30s) +Brdu (10min) cells using N6-methyladenosine/ m6A Rabbit monoclonal antibody (STJ11101784) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • The m6A rabbit monoclonal antibody (STJ11101784) are tested in Dot Blot against N6-methyladenosine (m6A) and unmodified adenosine. Oligomer 8- ATAACTGG-m6A-CCGAATGGOligomer 9- ATAACTGGACCGAATGGOligomer 10- AAAAAAAAAAAAAAAA-biotin.

Anti-N6-Methyl-m6A antibody [S4MR] (STJ11101784)

SKU:
STJ11101784

€183.13 - €799.38
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Host: Rabbit
Applications: IF
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-N6-Methyl-Adenosine/m6A is suitable for use in Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Protein A
Dilution Range: DB 1:500-1:2000
MeRIP 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: Chemical compounds corresponding to N6-methyladenosine/m6A.
Immunogen Sequence: Email for sequence
Background Predicted to enable anaphase-promoting complex binding activity and ubiquitin ligase activator activity. Involved in anaphase-promoting complex-dependent catabolic process; mitotic G2 DNA damage checkpoint signaling; and positive regulation of protein metabolic process. Located in nuclear membrane and nucleoplasm. Colocalizes with anaphase-promoting complex.

Information sourced from Uniprot.org

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