• Various whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-MyoD1 (ABT-MYOD1) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: Hela Lane 2: U2OSPredicted band size: 35kDaObserved band size: 45kDa
  • Immunohistochemical analysis of paraffin-embedded Rhabdomyosarcoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citrate buffer of pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of MyoD1Antibody at 1:1000 dilution.

Anti-MYOD1 antibody (100-200 aa) [ABT-MYOD1] (STJ196793)

SKU:
STJ196793

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Myoblast determination protein 1 (100-200 aa) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT-MYOD1
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:100-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYOD1
Gene ID: 4654
Uniprot ID: MYOD1_HUMAN
Immunogen Region: 100-200 aa
Specificity: This antibody detects endogenous levels of human MyoD1. Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin section
Immunogen: Synthesized peptide derived from the human MyoD1 at the amino acid range 100-200
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Protein Name Myoblast Determination Protein 1
Class C Basic Helix-Loop-Helix Protein 1
Bhlhc1
Myogenic Factor 3
Myf-3
Database Links Reactome: R-HSA-525793
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Myoblast Determination Protein 1 antibody
Anti-Class C Basic Helix-Loop-Helix Protein 1 antibody
Anti-Bhlhc1 antibody
Anti-Myogenic Factor 3 antibody
Anti-Myf-3 antibody
Anti-MYOD1 antibody
Anti-BHLHC1 antibody
Anti-MYF3 antibody
Anti-MYOD antibody

Information sourced from Uniprot.org

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