• Western blot analysis of extracts of various cell lines, using MYO5B antibody (STJ111294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunofluorescence analysis of Human colon carcinoma using MYO5B antibody (STJ111294) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Human placenta using MYO5B antibody (STJ111294) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Human placenta using MYO5B antibody (STJ111294) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Human colon carcinoma using MYO5B antibody (STJ111294) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using MYO5B antibody (STJ111294) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.

Anti-MYO5B antibody (1440-1690) (STJ111294)

SKU:
STJ111294

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYO5B (1440-1690) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYO5B
Gene ID: 4645
Uniprot ID: MYO5B_HUMAN
Immunogen Region: 1440-1690
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1440-1690 of human MYO5B (NP_001073936.1).
Immunogen Sequence: AQALAQSERKRHELNRQVTV QRKEKDFQGMLEYHKEDEAL LIRNLVTDLKPQMLSGTVPC LPAYILYMCIRHADYTNDDL KVHSLLTSTINGIKKVLKKH NDDFEMTSFWLSNTCRLLHC LKQYSGDEGFMTQNTAKQNE HCLKNFDLTEYRQVLSDLSI QIYQQLIKIAEGVLQPMIVS AMLENESIQGLSGVKPTGYR KRSSSMADGDNSYCLEAIIR QMNAFHTVMCDQGLDPEII
Function May be involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation. Required in a complex with RAB11A and RAB11FIP2 for the transport of NPC1L1 to the plasma membrane. Together with RAB11A participates in CFTR trafficking to the plasma membrane and TF (transferrin) recycling in nonpolarized cells. Together with RAB11A and RAB8A participates in epithelial cell polarization. Together with RAB25 regulates transcytosis. Required for proper localization of bile salt export pump ABCB11 at the apical/canalicular plasma membrane of hepatocytes.
Protein Name Unconventional Myosin-Vb
Database Links Reactome: R-HSA-432040
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Unconventional Myosin-Vb antibody
Anti-MYO5B antibody
Anti-KIAA1119 antibody

Information sourced from Uniprot.org

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