• Immunofluorescence analysis of paraffin-embedded rat heart using MYH7 Rabbit polyclonal antibody (STJ11105427) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of paraffin-embedded mouse heart using MYH7 Rabbit polyclonal antibody (STJ11105427) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunofluorescence analysis of paraffin-embedded Human heart using MYH7 Rabbit polyclonal antibody (STJ11105427) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of MYH7 in paraffin-embedded Human skeletal muscle using MYH7 Rabbit polyclonal antibody (STJ11105427) at dilution of 1:50 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from wild type (WT) and 293T transfected with MYH7 Rabbit pAb, using MYH7 (STJ11105427) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-MYH7 antibody (1240-1340) (STJ11105427)

SKU:
STJ11105427

$151.88 - $705.38
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Myosin-7 (1240-1340) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYH7
Gene ID: 4625
Uniprot ID: MYH7_HUMAN
Immunogen Region: 1240-1340
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1240-1340 of human MYH7 (NP_000248.2).
Immunogen Sequence: KAKANLEKMCRTLEDQMNEH RSKAEETQRSVNDLTSQRAK LQTENGELSRQLDEKEALIS QLTRGKLTYTQQLEDLKRQL EEEVKAKNALAHALQSARHD C
Tissue Specificity Both wild type and variant Gln-403 are detected in skeletal muscle (at protein level).
Function Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.
Protein Name Myosin-7
Myosin Heavy Chain 7
Myosin Heavy Chain Slow Isoform
Myhc-Slow
Myosin Heavy Chain - Cardiac Muscle Beta Isoform
Myhc-Beta
Cellular Localisation Cytoplasm
Myofibril
Sarcomere
Thick Filaments Of The Myofibrils
Alternative Antibody Names Anti-Myosin-7 antibody
Anti-Myosin Heavy Chain 7 antibody
Anti-Myosin Heavy Chain Slow Isoform antibody
Anti-Myhc-Slow antibody
Anti-Myosin Heavy Chain - Cardiac Muscle Beta Isoform antibody
Anti-Myhc-Beta antibody
Anti-MYH7 antibody
Anti-MYHCB antibody

Information sourced from Uniprot.org

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