• Immunofluorescence analysis of C6 cells using MYEF2 Rabbit polyclonal antibody (STJ118274) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MYEF2 in paraffin-embedded Human liver cancer using MYEF2 Rabbit polyclonal antibody (STJ118274) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MYEF2 in paraffin-embedded Rat testis using MYEF2 Rabbit polyclonal antibody (STJ118274) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MYEF2 in paraffin-embedded Mouse brain using MYEF2 Rabbit polyclonal antibody (STJ118274) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MYEF2 Rabbit polyclonal antibody (STJ118274) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MYEF2 antibody (1-120) (STJ118274)

SKU:
STJ118274

£112.50 - £522.50
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MYEF2 (1-120) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MYEF2
Gene ID: 50804
Uniprot ID: MYEF2_HUMAN
Immunogen Region: 1-120
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human MYEF2 (NP_001288139.1).
Immunogen Sequence: MADANKAEVPGATGGDSPHL QPAEPPGEPRREPHPAEAEK QQPQHSSSSNGVKMENDESA KEEKSDLKEKSTGSKKANRF HPYSKDKNSGTGEKKGPNRN RVFISNIPYDMKWQAIKDLM
Function Transcriptional repressor of the myelin basic protein gene (MBP). Binds to the proximal MB1 element 5'-TTGTCC-3' of the MBP promoter. Its binding to MB1 and function are inhibited by PURA.
Protein Name Myelin Expression Factor 2
Mef-2
Myef-2
Mst156
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Myelin Expression Factor 2 antibody
Anti-Mef-2 antibody
Anti-Myef-2 antibody
Anti-Mst156 antibody
Anti-MYEF2 antibody
Anti-KIAA1341 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance