• STJ70667 (0. 2µg/ml) staining of HumanThymus (A) and (0. 1ug/ml) Human Spleen (B) and Mouse Thymus © lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70667 (0. 1µg/ml) staining of Human Lymph node 1 (A) + peptide (B) , and Lymph node 2 (C) + peptide (D) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70667 (4µg/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. These results could not been obtained after antigen retireval at pH6 with this batch of antibody.
  • STJ70667 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70667 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70667 Flow cytometric analysis of paraformaldehyde fixed Jurkat cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-MYD88 antibody (Internal) (STJ70667)

SKU:
STJ70667-100

£312.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MYD88 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Approx 28kDa band observed in Human Spleen lysates (calculated MW of 28.3kDa according to NP_001166039.2). Recommended concentration: 0.3-0.5µg/ml. Primary incubation1 hour at room te
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MYD88
Gene ID: 4615
Uniprot ID: MYD88_HUMAN
Immunogen Region: Internal
Accession Number: NP_001166038.2; NP_002459.3; NP_001166039..2; NP_001361717.1
Specificity: This antibody is expected to recognize reported isoforms 1, 2, 3 and 9.
Immunogen Sequence: IKYKAMKKEFP
Post Translational Modifications Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination. OTUD4 specifically hydrolyzes 'Lys-63'-linked polyubiquitinated MYD88. Deubiquitinated by USP3 that cleaves 'Lys-63'-linked ubiquitin chains leading to inhibition of MYD88-induced NF-kappa-B signaling. (Microbial infection) Ubiquitinated by human herpesvirus 8 (KSHV) protein RTA/ORF50, leading to proteasomal degradation ans suppression of TLR4 signaling pathway.
Function Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine.
Protein Name Myeloid Differentiation Primary Response Protein Myd88
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-1257604
Reactome: R-HSA-166058
Reactome: R-HSA-1810476
Reactome: R-HSA-209543
Reactome: R-HSA-3134963
Reactome: R-HSA-5602498
Reactome: R-HSA-5602680
Reactome: R-HSA-5603037
Reactome: R-HSA-5603041
Reactome: R-HSA-6811558
Reactome: R-HSA-9020702
Reactome: R-HSA-975110
Reactome: R-HSA-975138
Reactome: R-HSA-975155
Reactome: R-HSA-975871
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Myeloid Differentiation Primary Response Protein Myd88 antibody
Anti-MYD88 antibody

Information sourced from Uniprot.org

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