Host: | Goat |
Applications: | Pep-ELISA/WB/IHC/IF/FC |
Reactivity: | Human/Mouse/Rat/Dog |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description: | Goat polyclonal antibody anti-MYD88 (Internal) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | IHC-4-6µg/mlIF-Strong expression of the protein seen in the nuclei and cytoplasm of U2OS and Jurkat cells. 10µg/mlELISA-antibody detection limit dilution 1:16000. |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | MYD88 |
Gene ID: | 4615 |
Uniprot ID: | MYD88_HUMAN |
Immunogen Region: | Internal |
Accession Number: | NP_001166038.2; NP_002459.3; NP_001166039..2; NP_001361717.1 |
Specificity: | This antibody is expected to recognize reported isoforms 1, 2, 3 and 9. |
Immunogen Sequence: | IKYKAMKKEFP |
Post Translational Modifications | Ubiquitinated.undergoes 'Lys-63'-linked polyubiquitination. OTUD4 specifically hydrolyzes 'Lys-63'-linked polyubiquitinated MYD88. |
Function | Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. Upon TLR8 activation by GU-rich single-stranded RNA (GU-rich RNA) derived from viruses such as SARS-CoV-2, SARS-CoV and HIV-1, induces IL1B release through NLRP3 inflammasome activation. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. |
Protein Name | Myeloid Differentiation Primary Response Protein Myd88 |
Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-1257604Reactome: R-HSA-166058Reactome: R-HSA-1810476Reactome: R-HSA-209543Reactome: R-HSA-3134963Reactome: R-HSA-5602498Reactome: R-HSA-5602680Reactome: R-HSA-5603037Reactome: R-HSA-5603041Reactome: R-HSA-6811558Reactome: R-HSA-9020702Reactome: R-HSA-975110Reactome: R-HSA-975138Reactome: R-HSA-975155Reactome: R-HSA-975871 |
Cellular Localisation | CytoplasmNucleus |
Alternative Antibody Names | Anti-Myeloid Differentiation Primary Response Protein Myd88 antibodyAnti-MYD88 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance