• Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein, using Myc-Tag rabbit monoclonal antibody antibody (STJ11103708) at 1:120000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of extracts of normal 293T cells, 293T transfected with NLK Protein and 293T transfected with PTEN Protein, using Myc-Tag antibody (STJ11103708) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of HeLa cells (Left: HeLa cells overexpressing recombinant protein with Myc-Tag；Right: Negative control without Myc-Tag) use rabbit anti Myc-Tag monoclonal antibody (STJ11103708) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300ug extract cell lysate from 293T cells transfected with NLK expression vector containing a Myc-Tag with 3 Mu g Myc-Tag rabbit monoclonal antibody antibody (STJ11103708). Western blot was performed from the immunoprecipitate using Myc-Tag rabbit monoclonal antibody antibody (STJ11103708) at 1:5000 dilution.
  • Immunoprecipitation analysis of 200ug extracts of 293T cells transfected with NLK expression vector containing a Myc-Tag with 3 Mu g Myc-Tag rabbit monoclonal antibody (STJ11103708 1:100). Western blot was performed from the immunoprecipitate using Myc-Tag rabbit monoclonal antibody (STJ11103708) at 1:1000 dilution.

Anti-Myc-Tag antibody [S8MR] (STJ11103708)

SKU:
STJ11103708

£212.50 - £335.50
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Myc-Tag is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:10000-1:140000
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen: A synthetic peptide corresponding to Myc tag.
Immunogen Sequence: EQKLISEEDL
Background Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.

Information sourced from Uniprot.org

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