• Western blot analysis of extracts of normal 293T cells and 293T transfected with Myc-NLK protein, using Myc-Tag antibody (STJ11103699) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of HeLa-Myc-CandHeLa cells using rabbit anti Myc-Tag polyclonal antibody (STJ11103699) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g rabbit anti Myc-Tag polyclonal antibody antibody (STJ11103699). Western blot was performed from the immunoprecipitate using rabbit anti Myc-Tag polyclonal antibody antibody (STJ11103699) at a dilution of 1:50000.
  • Immunoprecipitation analysis of 300 Mu g extracts of 293T-NLK-Myc cells using 3 Mu g Myc-Tag antibody (STJ11103699). Western blot was performed from the immunoprecipitate using Myc-Tag antibody (STJ11103699) at a dilution of 1:20000.

Anti-Myc-Tag antibody (C-Term) (STJ11103699)

SKU:
STJ11103699

£135.50 - £249.50
Current Stock:
Host: Rabbit
Applications: WB/IF/ICC/IP
Reactivity: Species independent
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:2000-1:6000
IF/ICC:1:50-1:200
IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Immunogen Region: C-Term
Specificity: A synthetic peptide corresponding to Myc tag.
Immunogen Sequence: EQKLISEEDL
Background Protein tags are peptide sequences genetically grafted onto a recombinant protein. Often these tags are removable by chemical agents or by enzymatic means, such as proteolysis or intein splicing. Tags are attached to proteins for various purposes.Epitope tags are short peptide sequences which are chosen because high-affinity antibodies can be reliably produced in many different species. These are usually derived from viral genes, which explain their high immunoreactivity. Epitope tags include V5-tag, Myc-tag, HA-tag and NE-tag. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification.

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance