| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MMUT |
| Gene ID: | 4594 |
| Uniprot ID: | MUTA_HUMAN |
| Immunogen Region: | 451-750 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 451-750 of human MUT (NP_000246.2). |
| Immunogen Sequence: | EMGGMAKAVAEGIPKLRIEE CAARRQARIDSGSEVIVGVN KYQLEKEDAVEVLAIDNTSV RNRQIEKLKKIKSSRDQALA ERCLAALTECAASGDGNILA LAVDASRARCTVGEITDALK KVFGEHKANDRMVSGAYRQE FGESKEITSAIKRVHKFMER EGRRPRLLVAKMGQDGHDRG AKVIATGFADLGFDVDIGPL FQTPREVAQQAVDADVHAVG ISTLAAGHKTLVPELIKEL |
| Function | Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle. |
| Protein Name | Methylmalonyl-Coa Mutase - MitochondrialMcmMethylmalonyl-Coa Isomerase |
| Database Links | Reactome: R-HSA-3359475Reactome: R-HSA-3359478Reactome: R-HSA-71032Reactome: R-HSA-9759218 |
| Cellular Localisation | Mitochondrion MatrixMitochondrionCytoplasm |
| Alternative Antibody Names | Anti-Methylmalonyl-Coa Mutase - Mitochondrial antibodyAnti-Mcm antibodyAnti-Methylmalonyl-Coa Isomerase antibodyAnti-MMUT antibodyAnti-MUT antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and STIM1 knockout (KO) HeLa cells, using [KO Validated] STIM1 Rabbit polyclonal antibody (STJ11100770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89707/358189/STJ11100770_1__47615.1713122504.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and STIM1 knockout (KO) HeLa cells, using [KO Validated] STIM1 Rabbit polyclonal antibody (STJ11100770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
