| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IHC-P:1:50-1:100ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MUS81 |
| Gene ID: | 80198 |
| Uniprot ID: | MUS81_HUMAN |
| Immunogen Region: | 1-350 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human MUS81 (NP_079404.3). |
| Immunogen Sequence: | MAAPVRLGRKRPLPACPNPL FVRWLTEWRDEATRSRRRTR FVFQKALRSLRRYPLPLRSG KEAKILQHFGDGLCRMLDER LQRHRTSGGDHAPDSPSGEN SPAPQGRLAEVQDSSMPVPA QPKAGGSGSYWPARHSGARV ILLVLYREHLNPNGHHFLTK EELLQRCAQKSPRVAPGSAR PWPALRSLLHRNLVLRTHQP ARYSLTPEGLELAQKLAESE GLSLLNVGIGPKEPPGEET |
| Tissue Specificity | Widely expressed. |
| Function | Interacts with EME1 and EME2 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. Plays an essential role in mitosis for the processing of stalled or collapsed replication forks. |
| Protein Name | Crossover Junction Endonuclease Mus81 |
| Database Links | Reactome: R-HSA-5693568Reactome: R-HSA-6783310 |
| Cellular Localisation | NucleusNucleolusRecruited To Foci Of Dna Damage In S-Phase Cells |
| Alternative Antibody Names | Anti-Crossover Junction Endonuclease Mus81 antibodyAnti-MUS81 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and STRAP knockout (KO) 293T cells, using [KO Validated] STRAP Rabbit polyclonal antibody (STJ11100865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89799/358267/STJ11100865_1__02334.1713122609.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and STRAP knockout (KO) 293T cells, using [KO Validated] STRAP Rabbit polyclonal antibody (STJ11100865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
