• STJ70333 (2µg/ml) staining of HeLa cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
  • STJ70333 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70333 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-MURF2/TRIM55 antibody (C-Term) (STJ70333)

SKU:
STJ70333-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human/Mouse/Rat/Dog/Pig
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-MURF2/TRIM55 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-1-3µg/ml
IHC-10µg/ml
IF-Strong expression of the protein seen in the Golgi and cytoplasm of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TRIM55
Gene ID: 84675
Uniprot ID: TRI55_HUMAN
Immunogen Region: C-Term
Accession Number: NP_908973.1; NP_908974.1; NP_908975.1
Specificity: This antibody is expected to recognize isoforms 1, 3 and 4 (NP_908973.1, NP_908974.1and NP_908975.1 resp.).
Immunogen Sequence: HIFSFSWLNSLNE
Function May regulate gene expression and protein turnover in muscle cells.
Protein Name Tripartite Motif-Containing Protein 55
Muscle-Specific Ring Finger Protein 2
Murf-2
Murf2
Ring Finger Protein 29
Cellular Localisation Cytoplasm
Nucleus
Nuclear Under Atrophic Conditions And Upon Mechanical Signals
Localizes To The Sarcomeric M-Band In Cardiomyocytes
Colocalizes In Part With Microtubules
Alternative Antibody Names Anti-Tripartite Motif-Containing Protein 55 antibody
Anti-Muscle-Specific Ring Finger Protein 2 antibody
Anti-Murf-2 antibody
Anti-Murf2 antibody
Anti-Ring Finger Protein 29 antibody
Anti-TRIM55 antibody
Anti-MURF2 antibody
Anti-RNF29 antibody

Information sourced from Uniprot.org

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