• STJ72478 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ72478-Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing vesicle staining. The nuclear stain is DAPI (blue). NA NA NA Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-Muc5b-Mouse antibody (Internal) (STJ72478)

SKU:
STJ72478-100

$421.88
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Host: Goat
Applications: Pep-ELISA/IF
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Muc5b-Mouse (Internal) is suitable for use in ELISA and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: Peptide ELISA: antibody detection limit dilution 1:128000.
WB: Not yet tested-our routine western blotting protocol does not allow for the detection of proteins >250kDa.
IF: Strong expression of the protein seen in vesicles of NIH3T3 cells. R
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: MUC5B
Gene ID: 727897
Uniprot ID: MUC5B_HUMAN
Immunogen Region: Internal
Accession Number: NP_002449.1
Immunogen Sequence: QHTYTHIDECN
Post Translational Modifications Highly glycosylated. C-, N- and O-glycosylated. C-mannosylated in the Cys-rich subdomains probably on the first Trp residue of the WXXW motif. Highly O-glycosylated in the Ser/Thr-rich tandem repeat (TR) region. The repeat region is about 59% O-glycosylated with a high abundance of NeuAc(2)Hex(1)HexNac1-ol.
Function Gel-forming mucin that is thought to contribute to the lubricating and viscoelastic properties of whole saliva and cervical mucus.
Protein Name Mucin-5b
Muc-5b
Cervical Mucin
High Molecular Weight Salivary Mucin Mg1
Mucin-5 Subtype B - Tracheobronchial
Sublingual Gland Mucin
Database Links Reactome: R-HSA-5083625
Reactome: R-HSA-5083632
Reactome: R-HSA-5083636
Reactome: R-HSA-5621480
Reactome: R-HSA-913709
Reactome: R-HSA-977068
Cellular Localisation Secreted
Alternative Antibody Names Anti-Mucin-5b antibody
Anti-Muc-5b antibody
Anti-Cervical Mucin antibody
Anti-High Molecular Weight Salivary Mucin Mg1 antibody
Anti-Mucin-5 Subtype B - Tracheobronchial antibody
Anti-Sublingual Gland Mucin antibody
Anti-MUC5B antibody
Anti-MUC5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance