| Applications: | WB/IHC-P/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:1000-1:5000IHC-P:1:100-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MTNR1A |
| Gene ID: | 4543 |
| Uniprot ID: | MTR1A_HUMAN |
| Immunogen Region: | 281-350 |
| Immunogen Sequence: | YYMAYFNSCLNAIIYGLLNQ NFRKEYRRIIVSLCTARVFF VDSSNDVADRVKWKPSPLMT NNNVVKVDSV |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 281-350 of human MTNR1A (NP_005949.1). |
| Tissue Specificity | Expressed in hypophyseal pars tuberalis and hypothalamic suprachiasmatic nuclei (SCN). Hippocampus. |
| Function | High affinity receptor for melatonin. Likely to mediate the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity. Possibly involved in sleep induction, by melatonin activation of the potassium channel KCNMA1/BK and the dissociation of G-beta and G-gamma subunits, thereby decreasing synaptic transmission. |
| Protein Name | Melatonin Receptor Type 1aMel-1a-RMel1a Receptor |
| Database Links | Reactome: R-HSA-373076Reactome: R-HSA-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Melatonin Receptor Type 1a antibodyAnti-Mel-1a-R antibodyAnti-Mel1a Receptor antibodyAnti-MTNR1A antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and PTPN1 knockout (KO) HeLa (KO) cells, using [KO Validated] PTPN1 Rabbit polyclonal antibody (STJ11104770) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/177414/369143/STJ11104770_1__28954.1713134627.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PTPN1 knockout (KO) HeLa (KO) cells, using [KO Validated] PTPN1 Rabbit polyclonal antibody (STJ11104770) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
