• Immunofluorescence analysis of L929 cells using MT-CYB Rabbit polyclonal antibody (STJ119945) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MT-CYB in paraffin-embedded human thyroid cancer tissue using MT-CYB Rabbit polyclonal antibody (STJ119945) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MT-CYB in paraffin-embedded rat liver tissue using MT-CYB Rabbit polyclonal antibody (STJ119945) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MT-CYB in paraffin-embedded mouse kidney tissue using MT-CYB Rabbit polyclonal antibody (STJ119945) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using MT-CYB Rabbit polyclonal antibody (STJ119945) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-MT-CYB antibody (100-200) (STJ119945)

SKU:
STJ119945

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:100
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MT-CYB
Gene ID: 4519
Uniprot ID: CYB_HUMAN
Immunogen Region: 100-200
Specificity: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MT-CYB (YP_003024038.1).
Immunogen Sequence: RGLYYGSFLYSETWNIGIIL LLATMATAFMGYVLPWGQMS FWGATVITNLLSAIPYIGTD LVQWIWGGYSVDSPTLTRFF TFHFILPFIIAALATLHLLF L
Function Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.
Protein Name Cytochrome B
Complex Iii Subunit 3
Complex Iii Subunit Iii
Cytochrome B-C1 Complex Subunit 3
Ubiquinol-Cytochrome-C Reductase Complex Cytochrome B Subunit
Database Links Reactome: R-HSA-611105
Reactome: R-HSA-9865881
Cellular Localisation Mitochondrion Inner Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Cytochrome B antibody
Anti-Complex Iii Subunit 3 antibody
Anti-Complex Iii Subunit Iii antibody
Anti-Cytochrome B-C1 Complex Subunit 3 antibody
Anti-Ubiquinol-Cytochrome-C Reductase Complex Cytochrome B Subunit antibody
Anti-MT-CYB antibody
Anti-COB antibody
Anti-CYTB antibody
Anti-MTCYB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance