| Host: | Rabbit |
| Applications: | WB/IHC-P/IF/ICC/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S8MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:1000-1:6000 IHC-P:1:200-1:800 IF/ICC:1:200-1:800 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MT-CO2 |
| Gene ID: | 4513 |
| Uniprot ID: | COX2_HUMAN |
| Immunogen Region: | 128-227 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 128-227 of human MTCO2 (P00403). |
| Immunogen Sequence: | LEPGDLRLLDVDNRVVLPIE APIRMMITSQDVLHSWAVPT LGLKTDAIPGRLNQTTFTAT RPGVYYGQCSEICGANHSFM PIVLELIPLKIFEMGPVFTL |
| Function | Component of the cytochrome c oxidase, the last enzyme in the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Electrons originating from reduced cytochrome c in the intermembrane space (IMS) are transferred via the dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 to the active site in subunit 1, a binuclear center (BNC) formed by heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 water molecules using 4 electrons from cytochrome c in the IMS and 4 protons from the mitochondrial matrix. |
| Protein Name | Cytochrome C Oxidase Subunit 2 Cytochrome C Oxidase Polypeptide Ii |
| Database Links | Reactome: R-HSA-5628897 Reactome: R-HSA-611105 Reactome: R-HSA-9707564 Reactome: R-HSA-9837999 Reactome: R-HSA-9864848 |
| Cellular Localisation | Mitochondrion Inner Membrane Multi-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Cytochrome C Oxidase Subunit 2 antibody Anti-Cytochrome C Oxidase Polypeptide Ii antibody Anti-MT-CO2 antibody Anti-COII antibody Anti-COX2 antibody Anti-COXII antibody Anti-MTCO2 antibody |
Information sourced from Uniprot.org