• Confocal imaging of paraffin-embedded Rat liver tissue using MSP/MST1 Rabbit monoclonal antibody (STJ11101227, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Human liver tissue using MSP/MST1 Rabbit monoclonal antibody (STJ11101227, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of MSP/MST1 in paraffin-embedded mouse testis tissue using MSP/MST1 Rabbit monoclonal antibody (STJ11101227) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MSP/MST1 in paraffin-embedded mouse kidney tissue using MSP/MST1 Rabbit monoclonal antibody (STJ11101227) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MSP/MST1 in paraffin-embedded mouse intestin tissue using MSP/MST1 Rabbit monoclonal antibody (STJ11101227) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using MSP/MST1 Rabbit monoclonal antibody (STJ11101227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-MST1 antibody (1-100) [S7MR] (STJ11101227)

SKU:
STJ11101227

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MST1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S7MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MST1
Gene ID: 4485
Uniprot ID: HGFL_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSP/MST1 (NP_066278.3).
Immunogen Sequence: MGWLPLLLLLTQCLGVPGQR SPLNDFQVLRGTELQHLLHA VVPGPWQEDVADAEECAGRC GPLMDCRAFHYNVSSHGCQL LPWTQHSPHTRLRRSGRCDL
Post Translational Modifications Cleaved after Arg-483, probably by HPN/Hepsin, to yield the active form consisting of two disulfide-linked chains.
Protein Name Hepatocyte Growth Factor-Like Protein
Macrophage Stimulatory Protein
Macrophage-Stimulating Protein
Msp Cleaved Into - Hepatocyte Growth Factor-Like Protein Alpha Chain - Hepatocyte Growth Factor-Like Protein Beta Chain
Database Links Reactome: R-HSA-8852405
Cellular Localisation Secreted
Alternative Antibody Names Anti-Hepatocyte Growth Factor-Like Protein antibody
Anti-Macrophage Stimulatory Protein antibody
Anti-Macrophage-Stimulating Protein antibody
Anti-Msp Cleaved Into - Hepatocyte Growth Factor-Like Protein Alpha Chain - Hepatocyte Growth Factor-Like Protein Beta Chain antibody
Anti-MST1 antibody
Anti-D3F15S2 antibody
Anti-DNF15S2 antibody
Anti-HGFL antibody

Information sourced from Uniprot.org

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