• Anti-MSH6 antibody [5B11] (STJ98124)
  • Western blot analysis using GTBP monoclonal antibody against MCF-7 (1) , HEK293 (2) , and HCT116 (3) cell lysate.

Anti-MSH6 antibody [5B11] (STJ98124)

SKU:
STJ98124

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-DNA mismatch repair protein Msh6 is suitable for use in Western Blot and ELISA research applications.
Clonality: Monoclonal
Clone ID: 5B11
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MSH6
Gene ID: 2956
Uniprot ID: MSH6_HUMAN
Specificity: GTBP Monoclonal Antibody detects endogenous levels of GTBP protein.
Immunogen: Purified recombinant fragment of human GTBP expressed in E. Coli.
Function Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.
Protein Name Dna Mismatch Repair Protein Msh6
Hmsh6
G/T Mismatch-Binding Protein
Gtbp
Gtmbp
Muts Protein Homolog 6
Muts-Alpha 160 Kda Subunit
P160
Database Links Reactome: R-HSA-5358565
Reactome: R-HSA-5632928
Reactome: R-HSA-5632968
Cellular Localisation Nucleus
Chromosome
Associates With H3k36me3 Via Its Pwwp Domain
Alternative Antibody Names Anti-Dna Mismatch Repair Protein Msh6 antibody
Anti-Hmsh6 antibody
Anti-G/T Mismatch-Binding Protein antibody
Anti-Gtbp antibody
Anti-Gtmbp antibody
Anti-Muts Protein Homolog 6 antibody
Anti-Muts-Alpha 160 Kda Subunit antibody
Anti-P160 antibody
Anti-MSH6 antibody
Anti-GTBP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance