• Immunofluorescence analysis of L929 cells using MRPL45 Rabbit polyclonal antibody (STJ115163) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of Hela cells using MRPL45 Rabbit polyclonal antibody (STJ115163) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MRPL45 in paraffin-embedded human breast cancer using MRPL45 Rabbit polyclonal antibody (STJ115163) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MRPL45 Rabbit polyclonal antibody (STJ115163) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MRPL45 antibody (57-306) (STJ115163)

SKU:
STJ115163

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL45
Uniprot ID: RM45_HUMAN
Immunogen Region: 57-306
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 57-306 of human MRPL45 (NP_115727.5).
Immunogen Sequence: MQHARKAGLVIPPEKSDRSI HLACTAGIFDAYVPPEGDAR ISSLSKEGLIERTERMKKTM ASQVSIRRIKDYDANFKIKD FPEKAKDIFIEAHLCLNNSD HDRLHTLVTEHCFPDMTWDI KYKTVRWSFVESLEPSHVVQ VRCSSMMNQGNVYGQITVRM HTRQTLAIYDRFGRLMYGQE DVPKDVLEYVVFEKQLTNPY GSWRMHTKIVPPWAPPKQPI LKTVMIPGPQLKPEEEYEE
Function Component of the mitochondrial large ribosomal subunit (mt-LSU). Within the mitochondrial ribosomes, required to direct the nascent polypeptide toward the tunnel exit and position the exit at a distance from the membrane surface.
Protein Name Large Ribosomal Subunit Protein Ml45
39s Ribosomal Protein L45 - Mitochondrial
L45mt
Mrp-L45
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Ml45 antibody
Anti-39s Ribosomal Protein L45 - Mitochondrial antibody
Anti-L45mt antibody
Anti-Mrp-L45 antibody
Anti-MRPL45 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance