| Post Translational Modifications | Two mature forms are produced by differential two-step proteolytic cleavage. Cleaved by the mitochondrial processing protease to produce the long mature form and subsequently by the mitochondrial intermediate protease to produce the short mature form. In the presence of CUL3, undergoes 'Lys-63'-linked ubiquitination at Lys-150 which results in proteasomal degradation. |
| Function | As a component of the mitochondrial large ribosomal subunit, plays a role in mitochondrial translation. When present in mitochondria as a free protein not associated with the ribosome, associates with mitochondrial RNA polymerase POLRMT to activate transcription. Required for POLRMT stability. |
| Protein Name | Large Ribosomal Subunit Protein Bl12m39s Ribosomal Protein L12 - MitochondrialL12mtMrp-L125c5-2 Cleaved Into - Large Ribosomal Subunit Protein Bl12m - Long Mature Form - Large Ribosomal Subunit Protein Bl12m - Short Mature Form |
| Database Links | Reactome: R-HSA-5368286Reactome: R-HSA-5389840Reactome: R-HSA-5419276Reactome: R-HSA-9837999 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Large Ribosomal Subunit Protein Bl12m antibodyAnti-39s Ribosomal Protein L12 - Mitochondrial antibodyAnti-L12mt antibodyAnti-Mrp-L12 antibodyAnti-5c5-2 Cleaved Into - Large Ribosomal Subunit Protein Bl12m - Long Mature Form - Large Ribosomal Subunit Protein Bl12m - Short Mature Form antibodyAnti-MRPL12 antibodyAnti-MRPL7 antibodyAnti-RPML12 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and SRI knockout (KO) HeLa cells, using [KO Validated] SRI Rabbit polyclonal antibody (STJ11100797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89733/358211/STJ11100797_1__02864.1713122535.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SRI knockout (KO) HeLa cells, using [KO Validated] SRI Rabbit polyclonal antibody (STJ11100797) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
