• Western blot analysis of extracts of various cell lines, using MRPL12 antibody (STJ110616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
  • Immunohistochemistry analysis of MRPL12 in paraffin-embedded human thyroid cancer tissue using MRPL12 Rabbit polyclonal antibody (STJ110616) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using MRPL12 Rabbit polyclonal antibody (STJ110616) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MRPL12 antibody (1-198) (STJ110616)

SKU:
STJ110616

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MRPL12 (1-198) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRPL12
Gene ID: 6182
Uniprot ID: RM12_HUMAN
Immunogen Region: 1-198
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2).
Immunogen Sequence: MLPAAARPLWGPCLGLRAAA FRLARRQVPCVCAVRHMRSS GHQRCEALAGAPLDNAPKEY PPKIQQLVQDIASLTLLEIS DLNELLKKTLKIQDVGLVPM GGVMSGAVPAAAAQEAVEED IPIAKERTHFTVRLTEAKPV DKVKLIKEIKNYIQGINLVQ AKKLVESLPQEIKANVAKAE AEKIKAALEAVGGTVVLE
Function As a component of the mitochondrial large ribosomal subunit, it plays a role in mitochondrial translation. Associates with mitochondrial RNA polymerase to activate transcription.
Protein Name Large Ribosomal Subunit Protein Bl12m
39s Ribosomal Protein L12 - Mitochondrial
L12mt
Mrp-L12
5c5-2
Database Links Reactome: R-HSA-5368286
Reactome: R-HSA-5389840
Reactome: R-HSA-5419276
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Large Ribosomal Subunit Protein Bl12m antibody
Anti-39s Ribosomal Protein L12 - Mitochondrial antibody
Anti-L12mt antibody
Anti-Mrp-L12 antibody
Anti-5c5-2 antibody
Anti-MRPL12 antibody
Anti-MRPL7 antibody
Anti-RPML12 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance