• Western blot analysis of lysates from wild type (WT) and MPG knockout (KO) HeLa cells, using [KO Validated] MPG Rabbit polyclonal antibody (STJ11100043) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MPG antibody (1-293) (STJ11100043)

SKU:
STJ11100043

£129.50 - £639.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MPG (1-293) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MPG
Gene ID: 4350
Uniprot ID: 3MG_HUMAN
Immunogen Region: 1-293
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-293 of human MPG (NP_001015052.1).
Immunogen Sequence: MPARSGAQFCRRMGQKKQRP ARAGQPHSSSDAAQAPAEQP HSSSDAAQAPCPRERCLGPP TTPGPYRSIYFSSPKGHLTR LGLEFFDQPAVPLARAFLGQ VLVRRLPNGTELRGRIVETE AYLGPEDEAAHSRGGRQTPR NRGMFMKPGTLYVYIIYGMY FCMNISSQGDGACVLLRALE PLEGLETMRQLRSTLRKGTA SRVLKDRELCSGPSKLCQAL AINKSFDQRDLAQDEAVWL
Function Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Protein Name Dna-3-Methyladenine Glycosylase
3-Alkyladenine Dna Glycosylase
3-Methyladenine Dna Glycosidase
Adpg
N-Methylpurine-Dna Glycosylase
Database Links Reactome: R-HSA-110330
Reactome: R-HSA-110331
Reactome: R-HSA-110357
Cellular Localisation Cytoplasm
Mitochondrion Matrix
Mitochondrion Nucleoid
Nucleus
Alternative Antibody Names Anti-Dna-3-Methyladenine Glycosylase antibody
Anti-3-Alkyladenine Dna Glycosylase antibody
Anti-3-Methyladenine Dna Glycosidase antibody
Anti-Adpg antibody
Anti-N-Methylpurine-Dna Glycosylase antibody
Anti-MPG antibody
Anti-AAG antibody
Anti-ANPG antibody
Anti-MID1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance