• Immunofluorescence analysis of L929 cells using MMP25 antibody (STJ24585) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using MMP25 antibody (STJ24585) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using MMP25 antibody (STJ24585) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using MMP25 antibody (STJ24585) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-MMP25 antibody (350-540) (STJ24585)

SKU:
STJ24585

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MMP25 (350-540) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MMP25
Gene ID: 64386
Uniprot ID: MMP25_HUMAN
Immunogen Region: 350-540
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 350-540 of human MMP25 (NP_071913.1).
Immunogen Sequence: SPRPARLHRFWEGLPAQVRV VQAAYARHRDGRILLFSGPQ FWVFQDRQLEGGARPLTELG LPPGEEVDAVFSWPQNGKTY LVRGRQYWRYDEAAARPDPG YPRDLSLWEGAPPSPDDVTV SNAGDTYFFKGAHYWRFPKN SIKTEPDAPQPMGPNWLDCP APSSGPRAPRPPKATPVSET CDCQCELNQAA
Tissue Specificity Expressed predominantly in leukocytes, lung and spleen. Expressed also in colon carcinoma, astrocytoma and glioblastomas.
Post Translational Modifications The precursor is cleaved by a furin endopeptidase.
Function May activate progelatinase A.
Protein Name Matrix Metalloproteinase-25
Mmp-25
Leukolysin
Membrane-Type Matrix Metalloproteinase 6
Mt-Mmp 6
Mtmmp6
Membrane-Type-6 Matrix Metalloproteinase
Mt6-Mmp
Mt6mmp
Database Links Reactome: R-HSA-1592389
Reactome: R-HSA-6798695
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Secreted
Extracellular Space
Extracellular Matrix
Alternative Antibody Names Anti-Matrix Metalloproteinase-25 antibody
Anti-Mmp-25 antibody
Anti-Leukolysin antibody
Anti-Membrane-Type Matrix Metalloproteinase 6 antibody
Anti-Mt-Mmp 6 antibody
Anti-Mtmmp6 antibody
Anti-Membrane-Type-6 Matrix Metalloproteinase antibody
Anti-Mt6-Mmp antibody
Anti-Mt6mmp antibody
Anti-MMP25 antibody
Anti-MMP20 antibody
Anti-MMPL1 antibody
Anti-MT6MMP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance