Anti-MLH1 antibody (400-500 aa) [ABT-MLH1] (STJ196792)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
IsotypeIgG1k
ImmunogenSynthesized peptide derived from the human MutL Protein Homolog 1 (MLH1) at the amino acid range 400-500
General Information
| Short Description | Mouse monoclonal anti-DNA mismatch repair protein Mlh1 (400-500 aa) for use in WB, IHC and IF in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF |
| Host | Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | ABT-MLH1 |
| Isotype | IgG1k |
| Conjugation | Unconjugated |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | IHC-P 1:100-500WB 1:100-2000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MLH1 |
| Gene ID | 4292 |
| Uniprot ID | MLH1_HUMAN |
| Immunogen | Synthesized peptide derived from the human MutL Protein Homolog 1 (MLH1) at the amino acid range 400-500 |
| Immunogen Region | 400-500 aa |
| Specificity | This antibody detects endogenous levels of human MutL Protein Homolog 1 (MLH1). Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin |
Additional Info
| Post Translational Modifications | Acetylated. Deacetylated by HDAC6 which prevents the MutL alpha complex, formed by the MLH1-PMS2 heterodimer, from being recruited to the MutS alpha complex, formed by the MSH2-MSH6 heterodimer, leading to tolerance of DNA damage. Ubiquitinated by UBR4.leading to proteasomal degradation. This ubiquitination is counteracted by the deubiquitinase USP5. |
| Function | Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis. |
| Protein Name | Dna Mismatch Repair Protein Mlh1Mutl Protein Homolog 1 |
| Database Links | Reactome: R-HSA-5358565Reactome: R-HSA-5358606Reactome: R-HSA-5545483Reactome: R-HSA-5632987Reactome: R-HSA-6796648Reactome: R-HSA-912446 |
| Cellular Localisation | NucleusChromosomeRecruited To Chromatin In A Mcm9-Dependent Manner |
| Alternative Antibody Names | Anti-Dna Mismatch Repair Protein Mlh1 antibodyAnti-Mutl Protein Homolog 1 antibodyAnti-MLH1 antibodyAnti-COCA2 antibody |
Information sourced from Uniprot.org