• Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-MLH1 (ABT-MLH1) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562 Lane 2: HEK293 Lane 3: HelaPredicted band size: 84kDaObserved band size: 84kDa
  • Human rectal carcinoma tissue was stained with Anti-MLH1 (ABT-MLH1) Antibody
  • Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Citric acid, pH6.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Western blot analysis of MLH1Antibody at 1:1000 dilution.

Anti-MLH1 antibody (400-500 aa) [ABT-MLH1] (STJ196792)

SKU:
STJ196792

£168.00 - £226.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-DNA mismatch repair protein Mlh1 (400-500 aa) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: ABT-MLH1
Conjugation: Unconjugated
Isotype: IgG1k
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Dilution Range: IHC-P 1:100-500
WB 1:100-2000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MLH1
Gene ID: 4292
Uniprot ID: MLH1_HUMAN
Immunogen Region: 400-500 aa
Specificity: This antibody detects endogenous levels of human MutL Protein Homolog 1 (MLH1). Heat-induced epitope retrieval (HIER) Citrate buffer of pH6.0 was highly recommended as antigen repair method in paraffin
Immunogen: Synthesized peptide derived from the human MutL Protein Homolog 1 (MLH1) at the amino acid range 400-500
Function Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
Protein Name Dna Mismatch Repair Protein Mlh1
Mutl Protein Homolog 1
Database Links Reactome: R-HSA-5358565
Reactome: R-HSA-5358606
Reactome: R-HSA-5545483
Reactome: R-HSA-5632987
Reactome: R-HSA-6796648
Reactome: R-HSA-912446
Cellular Localisation Nucleus
Chromosome
Recruited To Chromatin In A Mcm9-Dependent Manner
Alternative Antibody Names Anti-Dna Mismatch Repair Protein Mlh1 antibody
Anti-Mutl Protein Homolog 1 antibody
Anti-MLH1 antibody
Anti-COCA2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance