• Western blot analysis of extracts of various cell lines, using MKS1 antibody (STJ26874) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-MKS1 antibody (150-300) (STJ26874)

SKU:
STJ26874

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MKS1 (150-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MKS1
Gene ID: 54903
Uniprot ID: MKS1_HUMAN
Immunogen Region: 150-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 150-300 of human MKS1 (NP_060247.2).
Immunogen Sequence: EVPSFLVERMANVRRRRQDR RGMEGGILKSRIVTWEPSEE FVRNNHVINTPLQTMHIMAD LGPYKKLGYKKYEHVLCTLK VDSNGVITVKPDFTGLKGPY RIETEGEKQELWKYTIDNVS PHAQPEEEERERRVFKDLYG RHKEYLSSLVG
Function Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in centrosome migration to the apical cell surface during early ciliogenesis. Required for ciliary structure and function, including a role in regulating length and appropriate number through modulating centrosome duplication. Required for cell branching morphology.
Protein Name Tectonic-Like Complex Member Mks1
Meckel Syndrome Type 1 Protein
Database Links Reactome: R-HSA-5610787
Reactome: R-HSA-5620912
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Basal Body
Microtubule Organizing Center
Centrosome
Localizes At The Transition Zone
A Region Between The Basal Body And The Ciliary Axoneme
Alternative Antibody Names Anti-Tectonic-Like Complex Member Mks1 antibody
Anti-Meckel Syndrome Type 1 Protein antibody
Anti-MKS1 antibody

Information sourced from Uniprot.org

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