• Immunohistochemistry analysis of MITD1 in paraffin-embedded Human liver using MITD1 Rabbit polyclonal antibody (STJ11100491) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MITD1 Rabbit polyclonal antibody (STJ11100491) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-MITD1 antibody (1-249) (STJ11100491)

SKU:
STJ11100491

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MITD1 (1-249) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MITD1
Gene ID: 129531
Uniprot ID: MITD1_HUMAN
Immunogen Region: 1-249
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human MITD1 (NP_620153.1).
Immunogen Sequence: MAKSGLRQDPQSTAAATVLK RAVELDSESRYPQALVCYQE GIDLLLQVLKGTKDNTKRCN LREKISKYMDRAENIKKYLD QEKEDGKYHKQIKIEENATG FSYESLFREYLNETVTEVWI EDPYIRHTHQLYNFLRFCEM LIKRPCKVKTIHLLTSLDEG IEQVQQSRGLQEIEESLRSH GVLLEVQYSSSIHDREIRFN NGWMIKIGRGLDYFKKPQSR FSLGYCDFDLRPCHETTVD
Function Required for efficient abscission at the end of cytokinesis, together with components of the ESCRT-III complex.
Protein Name Mit Domain-Containing Protein 1
Cellular Localisation Late Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Midbody
Membrane
During Cytokinesis
Recruited To The Midbody Via Interaction With Chmp1a
Interacts With Membranes Enriched In Phosphoinositides
Alternative Antibody Names Anti-Mit Domain-Containing Protein 1 antibody
Anti-MITD1 antibody

Information sourced from Uniprot.org

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