• Immunohistochemistry analysis of paraffin-embedded rat brain using MIP antibody (STJ24556) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human stomach using MIP antibody (STJ24556) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using MIP antibody (STJ24556) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using MIP antibody (STJ24556) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MIP antibody (50-150) (STJ24556)

SKU:
STJ24556

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MIP (50-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:100-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MIP
Gene ID: 4284
Uniprot ID: MIP_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MIP (NP_036196.1).
Immunogen Sequence: LALATLVQSVGHISGAHVNP AVTFAFLVGSQMSLLRAFCY MAAQLLGAVAGAAVLYSVTP PAVRGNLALNTLHPAVSVGQ ATTVEIFLTLQFVLCIFATY D
Tissue Specificity Expressed in the cortex and nucleus of the retina lens (at protein level). Major component of lens fiber gap junctions.
Post Translational Modifications Subject to partial proteolytic cleavage in the eye lens core. Partial proteolysis promotes interactions between tetramers from adjoining membranes. Fatty acylated at Met-1 and Lys-238. The acyl modifications, in decreasing order of ion abundance, are: oleoyl (C18:1) > palmitoyl (C16:0) > stearoyl (C18:0) > eicosenoyl (C20:1) > dihomo-gamma-linolenoyl (C20:3) > palmitoleoyl (C16:1) > eicosadienoyl (C20:2).
Function Water channel. Channel activity is down-regulated by CALM when cytoplasmic Ca(2+) levels are increased. May be responsible for regulating the osmolarity of the lens. Interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core. Plays a role in cell-to-cell adhesion and facilitates gap junction coupling.
Protein Name Lens Fiber Major Intrinsic Protein
Aquaporin-0
Mip26
Mp26
Database Links Reactome: R-HSA-432047
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Cell Junction
Gap Junction
Alternative Antibody Names Anti-Lens Fiber Major Intrinsic Protein antibody
Anti-Aquaporin-0 antibody
Anti-Mip26 antibody
Anti-Mp26 antibody
Anti-MIP antibody
Anti-AQP0 antibody

Information sourced from Uniprot.org

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