| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-MIB1 (560-860) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
MIB1 |
| Gene ID: |
57534 |
| Uniprot ID: |
MIB1_HUMAN |
| Immunogen Region: |
560-860 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 560-860 of human MIB1/DIP1 (NP_065825.1). |
| Immunogen Sequence: |
DSEGDTPLHDAISKKRDDIL AVLLEAGADVTITNNNGFNA LHHAALRGNPSAMRVLLSKL PRPWIVDEKKDDGYTALHLA ALNNHVEVAELLVHQGNANL DIQNVNQQTALHLAVERQHT QIVRLLVRAGAKLDIQDKDG DTPLHEALRHHTLSQLRQLQ DMQDVGKVDAAWEPSKNTLI MGLGTQGAEKKSAASIACFL AANGADLSIRNKKGQSPLDL CPDPNLCKALAKCHKEKVS |
| Tissue Specificity | Widely expressed at low level. Expressed at higher level in spinal cord, ovary, whole brain, and all specific brain regions examined. |
| Post Translational Modifications | Ubiquitinated.possibly via autoubiquitination. Ubiquitinated.this modification is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. Probably mediates ubiquitination and subsequent proteasomal degradation of DAPK1, thereby antagonizing anti-apoptotic effects of DAPK1 to promote TNF-induced apoptosis. Involved in ubiquitination of centriolar satellite CEP131, CEP290 and PCM1 proteins and hence inhibits primary cilium formation in proliferating cells. Mediates 'Lys-63'-linked polyubiquitination of TBK1, which probably participates in kinase activation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Mib1Dapk-Interacting Protein 1Dip-1Mind Bomb Homolog 1Ring-Type E3 Ubiquitin Transferase Mib1Zinc Finger Zz Type With Ankyrin Repeat Domain Protein 2 |
| Database Links | Reactome: R-HSA-2122948Reactome: R-HSA-2644606Reactome: R-HSA-2691232Reactome: R-HSA-2894862Reactome: R-HSA-2979096Reactome: R-HSA-9013507 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentriolar SatelliteCell MembraneLocalizes To The Plasma MembraneIt Is MitochondrialHowever Such Localization Remains UnclearDisplaced From Centriolar Satellites In Response To Cellular StressSuch As Ultraviolet Light (Uv) Radiation Or Heat Shock |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Mib1 antibodyAnti-Dapk-Interacting Protein 1 antibodyAnti-Dip-1 antibodyAnti-Mind Bomb Homolog 1 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Mib1 antibodyAnti-Zinc Finger Zz Type With Ankyrin Repeat Domain Protein 2 antibodyAnti-MIB1 antibodyAnti-DIP1 antibodyAnti-KIAA1323 antibodyAnti-ZZANK2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
