| Short Description : | Rabbit polyclonal E3 Ubiquitin-Protein Ligase Mgrn1 (1-538) antibody for use in WB, IF, ICC and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IF/ICC/ELISA |
| Host: | Rabbit |
| Reactivity: | Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MGRN1 |
| Gene ID: | 23295 |
| Uniprot ID: | MGRN1_HUMAN |
| Immunogen Region: | 1-538 |
| Immunogen Sequence: | MGSILSRRIAGVEDIDIQAN SAYRYPPKSGNYFASHFFMG GEKFDTPHPEGYLFGENMDL NFLGSRPVQFPYVTPAPHEP VKTLRSLVNIRKDSLRLVRY KDDADSPTEDGDKPRVLYSL EFTFDADARVAITIYCQASE EFLNGRAVYSPKSPSLQSET VHYKRGVSQQFSLPSFKIDF SEWKDDELNFDLDRGVFPVV IQAVVDEGDVVEVTGHAHVL LAAFEKHMDGSFSVKPLKQ |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-538 of human MGRN1 (O60291). |
| Post Translational Modifications | Autoubiquitinated in vitro. |
| Function | E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling. |
| Protein Name | E3 Ubiquitin-Protein Ligase Mgrn1Mahogunin Ring Finger Protein 1Ring Finger Protein 156Ring-Type E3 Ubiquitin Transferase Mgrn1 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | Early EndosomeThe Endosomal Localization Is Dependent On The Interaction With Tsg101Isoform 1: CytoplasmCytosolNucleusTranslocation From The Cytosol To The Nucleus Is Seen Only In The Presence Of Mc1r And Mc4rBut Not Tbxa2rExcluded From NucleoliIsoform 2: CytoplasmIsoform 3: CytoplasmCell MembraneIsoform 4: Cytoplasm |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Mgrn1 antibodyAnti-Mahogunin Ring Finger Protein 1 antibodyAnti-Ring Finger Protein 156 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Mgrn1 antibodyAnti-MGRN1 antibodyAnti-KIAA0544 antibodyAnti-RNF156 antibody |
Information sourced from Uniprot.org
