Anti-MGEA5 antibody (380-580) (STJ111534)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal MGEA5 (380-580) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | OGA |
| Gene ID | 10724 |
| Uniprot ID | OGA_HUMAN |
| Immunogen Region | 380-580 |
| Immunogen Sequence | YQEPIMSQGAALSGEPTTLT KEEEKKQPDEEPMDMVVEKQ EETDHKNDNQILSEIVEAKM AEELKPMDTDKESIAESKSP EMSMQEDCISDIAPMQTDEQ TNKEQFVPGPNEKPLYTAEP VTLEDLQLLADLFYLPYEHG PKGAQMLREFQWLRANSSVV SVNCKGKDSEKIEEWRSRAA KFEEMCGLVMGMFTRLSNCA N |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 380-580 of human MGEA5 (NP_001135906.1). |
Additional Info
| Tissue Specificity | Ubiquitous. Shows highest expression in the brain, placenta and pancreas. |
| Post Translational Modifications | Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other.cleavage does not decrease enzyme activity. |
| Function | Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Deglycosylates a large and diverse number of proteins, such as CRYAB, ELK1, GSDMD, LMNB1 and TAB1. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro). Does not bind acetyl-CoA and does not have histone acetyltransferase activity. Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1. |
| Protein Name | Protein O-GlcnacaseOgaBeta-N-AcetylglucosaminidaseBeta-N-AcetylhexosaminidaseBeta-HexosaminidaseMeningioma-Expressed Antigen 5N-Acetyl-Beta-D-GlucosaminidaseN-Acetyl-Beta-GlucosaminidaseNuclear Cytoplasmic O-Glcnacase And AcetyltransferaseNcoat |
| Database Links | |
| Cellular Localisation | Isoform 3: NucleusIsoform 1: Cytoplasm |
| Alternative Antibody Names | Anti-Protein O-Glcnacase antibodyAnti-Oga antibodyAnti-Beta-N-Acetylglucosaminidase antibodyAnti-Beta-N-Acetylhexosaminidase antibodyAnti-Beta-Hexosaminidase antibodyAnti-Meningioma-Expressed Antigen 5 antibodyAnti-N-Acetyl-Beta-D-Glucosaminidase antibodyAnti-N-Acetyl-Beta-Glucosaminidase antibodyAnti-Nuclear Cytoplasmic O-Glcnacase And Acetyltransferase antibodyAnti-Ncoat antibodyAnti-OGA antibodyAnti-HEXC antibodyAnti-KIAA0679 antibodyAnti-MEA5 antibodyAnti-MGEA5 antibody |
Information sourced from Uniprot.org