| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-METTL8 (1-140) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
METTL8 |
| Gene ID: |
79828 |
| Uniprot ID: |
METL8_HUMAN |
| Immunogen Region: |
1-140 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human METTL8 (NP_001308089.1). |
| Immunogen Sequence: |
MNMIWRNSISCLRLGKVPHR YQSGYHPVAPLGSRILTDPA KVFEHNMWDHMQWSKEEEAA ARKKVKENSAVRVLLEEQVK YEREASKYWDTFYKIHKNKF FKDRNWLLREFPEILPVDQK PEEKARESSWDHVKTSATNR |
| Function | Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of mitochondrial tRNA(Ser)(UCN) and tRNA(Thr). N(3)-methylcytidine methylation modification regulates mitochondrial translation efficiency and is required for activity of the respiratory chain. N(3)-methylcytidine methylation of mitochondrial tRNA(Ser)(UCN) requires the formation of N(6)-dimethylallyladenosine(37) (i6A37) by TRIT1 as prerequisite. May also mediate N(3)-methylcytidine modification of mRNAs. The existence of N(3)-methylcytidine modification on mRNAs is however unclear, and additional evidences are required to confirm the role of the N(3)-methylcytidine-specific mRNA methyltransferase activity of METTL8 in vivo. |
| Protein Name | Trna N(3-Methylcytidine Methyltransferase Mettl8 - MitochondrialMethyltransferase-Like Protein 8Mrna N(3-Methylcytidine Methyltransferase Mettl8 |
| Cellular Localisation | MitochondrionMitochondrial Protein: The Cytoplasmic Or Nuclear Localization Observed By Some Groups Is Either The Result Of An Incorrect Localization Caused By N-Terminal Tagging That Interferes With Mitochondrial TargetingOr Splice Isoforms That Lack The N-Terminal Mitochondrial Transit Sequence |
| Alternative Antibody Names | Anti-Trna N(3-Methylcytidine Methyltransferase Mettl8 - Mitochondrial antibodyAnti-Methyltransferase-Like Protein 8 antibodyAnti-Mrna N(3-Methylcytidine Methyltransferase Mettl8 antibodyAnti-METTL8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
