• Immunofluorescence analysis of U-251MG cells using METTL7B Rabbit polyclonal antibody (STJ29280) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HepG2 cells using METTL7B Rabbit polyclonal antibody (STJ29280) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using METTL7B antibody (STJ29280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Rat liver, using METTL7B antibody (STJ29280) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-METTL7B antibody (165-244) (STJ29280)

SKU:
STJ29280

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-METTL7B (165-244) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TMT1B
Gene ID: 196410
Uniprot ID: TMT1B_HUMAN
Immunogen Region: 165-244
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 165-244 of human METTL7B (NP_689850.2).
Immunogen Sequence: RPGGVLFFWEHVAEPYGSWA FMWQQVFEPTWKHIGDGCCL TRETWKDLENAQFSEIQMER QPPPLKWLPVGPHIMGKAVK
Function Thiol S-methyltransferase that catalyzes the transfer of a methyl group from S-adenosyl-l-methionine to hydrogen sulfide and other thiol compounds including dithiothreitol, 7alpha-thiospironolactone, L-penicillamine, and captopril.
Protein Name Thiol S-Methyltransferase Tmt1b
Methyltransferase-Like Protein 7b
Thiol S-Methyltransferase Mettl7b
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Lipid Droplet
Highly Concentrated In The Perinuclear Area Of The Endoplasmic Reticulum (Er) And Surrounding Lipid Droplets
May Be Associated With The Specific Regions Of The Lr That Form Lipid Droplets And Targeted To The Initial Deposits Of Lipids Where The Lipid Droplets Form
Alternative Antibody Names Anti-Thiol S-Methyltransferase Tmt1b antibody
Anti-Methyltransferase-Like Protein 7b antibody
Anti-Thiol S-Methyltransferase Mettl7b antibody
Anti-TMT1B antibody
Anti-METTL7B antibody
Anti-UNQ594 antibody
Anti-PRO1180 antibody

Information sourced from Uniprot.org

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