Anti-MEK2 antibody [R01-9J3] (STJA0036142)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human MEK2
General Information
| Short Description | Rabbit monoclonal anti-MEK2 for use in WB, IHC-P, ICC, IF, IP and FC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ICC/IF/IP/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R01-9J3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200IP 1:50FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | MAP2K2 |
| Gene ID | 5605 |
| Uniprot ID | MP2K2_HUMAN |
| Immunogen | A synthesized peptide derived from human MEK2 |
Additional Info
| Post Translational Modifications | MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). Phosphorylated by MAP2K1/MEK1. (Microbial infection) Acetylation of Ser-222 and Ser-226 by Yersinia YopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway. |
| Function | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner.by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation. |
| Protein Name | Dual Specificity Mitogen-Activated Protein Kinase Kinase 2Map Kinase Kinase 2Mapkk 2Erk Activator Kinase 2Mapk/Erk Kinase 2Mek 2 |
| Database Links | Reactome: R-HSA-112411Reactome: R-HSA-170968Reactome: R-HSA-445144Reactome: R-HSA-5210891Reactome: R-HSA-5673000Reactome: R-HSA-5674135Reactome: R-HSA-5674499Reactome: R-HSA-6802946Reactome: R-HSA-6802948Reactome: R-HSA-6802952Reactome: R-HSA-6802955Reactome: R-HSA-9649948Reactome: R-HSA-9652169Reactome: R-HSA-9656223 |
| Cellular Localisation | CytoplasmMembranePeripheral Membrane ProteinMembrane Localization Is Probably Regulated By Its Interaction With Ksr1 |
| Alternative Antibody Names | Anti-Dual Specificity Mitogen-Activated Protein Kinase Kinase 2 antibodyAnti-Map Kinase Kinase 2 antibodyAnti-Mapkk 2 antibodyAnti-Erk Activator Kinase 2 antibodyAnti-Mapk/Erk Kinase 2 antibodyAnti-Mek 2 antibodyAnti-MAP2K2 antibodyAnti-MEK2 antibodyAnti-MKK2 antibodyAnti-PRKMK2 antibody |
Information sourced from Uniprot.org