Western blot analysis of various lysates using MEIOC Rabbit pAb (STJ11105937) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-MEIOC antibody (1-270 aa) (STJ11105937)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11105937
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General Information

Short DescriptionRabbit polyclonal MEIOC (1-270 aa) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityMouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolMeioc
Gene ID268491
Uniprot IDMEIOC_MOUSE
Immunogen Region1-270 aa
Immunogen SequenceMEVSGGDTCRPRHPQGLREG PEPKVAAAAAAFRGSANRCW NLSVDTSNRLSDVFNSMMLT GSAPFYDCYKSQNEDNVDLR QTCTPLSSSTEYASSIDSSL FYAPWSTYGDDIKQPPSSQI SVKNRIQTERNDYGSETDLY GLVSNILEEQDKSQPYFAEG TCSSNLKSVWPMNTSRFVDH HDLLTEPKRPVDTSISQQAF YSGESVSAVEKQYLHNSSLT PQQKIDELYHGYTGLDLEE
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of mouse MEIOC (NP_001121048.1).

Additional Info

Tissue Specificity Expressed specifically in fetal ovary and postnatal and adult testes (at protein level). In adult testis expressed in spermatocytes, beginning in preleptotene and extending through most stages of meiotic prophase I, including leptotene, zygotene, and pachytene.
Function Is required for meiosis completion in both male and female germ cells. Confers stability to numerous meiotic mRNAs in gonads allowing proper initiation and progression into meiosis prophase I. The function may involve YTHDC2 and is independent of induction by retinoic acid (RA). Maintains an extended meiotic prophase I by properly promoting the transition from a mitotic to a meiotic cell cycle program by binding transcripts through its interaction with YTHDC2 that regulate the mitotic cell cycle.
Protein Name Meiosis-Specific Coiled-Coil Domain-Containing Protein Meioc
Meiosis-Specific With Coiled-Coil Domain Protein
Database Links
Cellular Localisation Cytoplasm
Nucleus
At Late Pachytene A Fraction Is Nuclear
Alternative Antibody Names Anti-Meiosis-Specific Coiled-Coil Domain-Containing Protein Meioc antibody
Anti-Meiosis-Specific With Coiled-Coil Domain Protein antibody
Anti-Meioc antibody
Anti-Gm1564 antibody

Information sourced from Uniprot.org

Citations

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