• Western blot analysis of extracts from wild type (WT) and ME1 knockdown (KD) MCF7 (KD) cells, using ME1 antibody (STJ11105005) at 1:400 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ME1 antibody (472-572) (STJ11105005)

SKU:
STJ11105005

Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Nadp-Dependent Malic Enzyme (472-572) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ME1
Gene ID: 4199
Uniprot ID: MAOX_HUMAN
Immunogen Region: 472-572
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 472-572 of human ME1. (NP_002386.1).
Immunogen Sequence: QITDNIFLTTAEVIAQQVSD KHLEEGRLYPPLNTIRDVSL KIAEKIVKDAYQEKTATVYP EPQNKEAFVRSQMYSTDYDQ ILPDCYSWPEEVQKIQTKVD Q
Tissue Specificity Expressed in all tissues tested including liver, placenta and white adipose tissue.
Function Catalyzes the oxidative decarboxylation of (S)-malate in the presence of NADP(+) and divalent metal ions, and decarboxylation of oxaloacetate.
Protein Name Nadp-Dependent Malic Enzyme
Nadp-Me
Malic Enzyme 1
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-70268
Reactome: R-HSA-9818025
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Nadp-Dependent Malic Enzyme antibody
Anti-Nadp-Me antibody
Anti-Malic Enzyme 1 antibody
Anti-ME1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance