| Host: | Mouse |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-Protein Mdm4 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 2D10F4 |
| Conjugation: | Unconjugated |
| Isotype: | IgG1 |
| Formulation: | Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol. |
| Purification: | Affinity purification |
| Dilution Range: | WB 1:500-1:2000IHC 1:200-1:1000IF 1:200-1:1000ELISA 1:10000 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MDM4 |
| Gene ID: | 4194 |
| Uniprot ID: | MDM4_HUMAN |
| Specificity: | MDMX Monoclonal Antibody detects endogenous levels of MDMX protein. |
| Immunogen: | Purified recombinant fragment of human MDMX expressed in E. Coli. |
| Post Translational Modifications | Phosphorylated. Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent. Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein. |
| Function | Along with MDM2, contributes to TP53 regulation. Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions. |
| Protein Name | Protein Mdm4Double Minute 4 ProteinMdm2-Like P53-Binding ProteinProtein MdmxP53-Binding Protein Mdm4 |
| Database Links | Reactome: R-HSA-2559580Reactome: R-HSA-2559585Reactome: R-HSA-5689880Reactome: R-HSA-6804756Reactome: R-HSA-6804757Reactome: R-HSA-6804760Reactome: R-HSA-69541 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Protein Mdm4 antibodyAnti-Double Minute 4 Protein antibodyAnti-Mdm2-Like P53-Binding Protein antibodyAnti-Protein Mdmx antibodyAnti-P53-Binding Protein Mdm4 antibodyAnti-MDM4 antibodyAnti-MDMX antibody |
Information sourced from Uniprot.org