• Immunohistochemistry analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using MDMX monoclonal antibody.
  • Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using MDMX monoclonal antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
  • Western blot analysis using MDMX monoclonal antibody against HeLa (1) , A549 (2) and A431 (3) cell lysate.

Anti-MDM4 antibody [2D10F4] (STJ98235)

SKU:
STJ98235

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/ELISA
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Protein Mdm4 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Monoclonal
Clone ID: 2D10F4
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MDM4
Gene ID: 4194
Uniprot ID: MDM4_HUMAN
Specificity: MDMX Monoclonal Antibody detects endogenous levels of MDMX protein.
Immunogen: Purified recombinant fragment of human MDMX expressed in E. Coli.
Post Translational Modifications Phosphorylated. Phosphorylation at Ser-367 promotes interaction with YWHAG and subsequent ubiquitination and degradation. Phosphorylation at Ser-342 also induces ubiquitination and degradation but to a lower extent. Ubiquitinated and degraded by MDM2. Deubiquitination by USP2 on the other hand stabilizes the MDM4 protein.
Function Along with MDM2, contributes to TP53 regulation. Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions.
Protein Name Protein Mdm4
Double Minute 4 Protein
Mdm2-Like P53-Binding Protein
Protein Mdmx
P53-Binding Protein Mdm4
Database Links Reactome: R-HSA-2559580
Reactome: R-HSA-2559585
Reactome: R-HSA-5689880
Reactome: R-HSA-6804756
Reactome: R-HSA-6804757
Reactome: R-HSA-6804760
Reactome: R-HSA-69541
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Protein Mdm4 antibody
Anti-Double Minute 4 Protein antibody
Anti-Mdm2-Like P53-Binding Protein antibody
Anti-Protein Mdmx antibody
Anti-P53-Binding Protein Mdm4 antibody
Anti-MDM4 antibody
Anti-MDMX antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance