| Host: | Goat |
| Applications: | Pep-ELISA/IHC |
| Reactivity: | Human/Mouse/Rat/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-MDG1/DNAJB9 (C-Term) is suitable for use in ELISA and Immunohistochemistry research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:4000. WB: Preliminary experiments gave no signal but low background in A459 and Human Skeletal Muscle extracts at up to 1µg/ml. IHC: In paraffin embedded Human Kidney shows ER staining in PCT |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | DNAJB9 |
| Gene ID: | 4189 |
| Uniprot ID: | DNJB9_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_036460.1 |
| Immunogen Sequence: | RGNMVTTYTDCSGQ |
| Function | Co-chaperone for Hsp70 protein HSPA5/BiP that acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response (UPR). J domain-containing co-chaperones stimulate the ATPase activity of Hsp70 proteins and are required for efficient substrate recognition by Hsp70 proteins. In the unstressed endoplasmic reticulum, interacts with the luminal region of ERN1/IRE1 and selectively recruits HSPA5/BiP: HSPA5/BiP disrupts the dimerization of the active ERN1/IRE1 luminal region, thereby inactivating ERN1/IRE1. Also involved in endoplasmic reticulum-associated degradation (ERAD) of misfolded proteins. Required for survival of B-cell progenitors and normal antibody production. |
| Protein Name | Dnaj Homolog Subfamily B Member 9 Endoplasmic Reticulum Dna J Domain-Containing Protein 4 Er-Resident Protein Erdj4 Erdj4 Microvascular Endothelial Differentiation Gene 1 Protein Mdg-1 |
| Database Links | Reactome: R-HSA-381038 |
| Cellular Localisation | Endoplasmic Reticulum Lumen |
| Alternative Antibody Names | Anti-Dnaj Homolog Subfamily B Member 9 antibody Anti-Endoplasmic Reticulum Dna J Domain-Containing Protein 4 antibody Anti-Er-Resident Protein Erdj4 antibody Anti-Erdj4 antibody Anti-Microvascular Endothelial Differentiation Gene 1 Protein antibody Anti-Mdg-1 antibody Anti-DNAJB9 antibody Anti-MDG1 antibody Anti-UNQ743 antibody Anti-PRO1471 antibody |
Information sourced from Uniprot.org