• Western blot analysis of extracts of various cell lines, using MCMBP antibody (STJ24530) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-MCMBP antibody (1-240) (STJ24530)

SKU:
STJ24530

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MCMBP (1-240) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCMBP
Gene ID: 79892
Uniprot ID: MCMBP_HUMAN
Immunogen Region: 1-240
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human MCMBP (NP_079110.1).
Immunogen Sequence: MPCGEDWLSHPLGIVQGFFA QNGVNPDWEKKVIEYFKEKL KENNAPKWVPSLNEVPLHYL KPNSFVKFRCMIQDMFDPEF YMGVYETVNQNTKAHVLHFG KYRDVAECGPQQELDLNSPR NTTLERQTFYCVPVPGESTW VKEAYVNANQARVSPSTSYT PSRHKRSYEDDDDMDLQPNK QKDQHAGARQAGSVGGLQWC GEPKRLETEASTGQQLNSLN LSSPFDLNFPLPGEKGPAC
Function Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP.probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion.
Protein Name Mini-Chromosome Maintenance Complex-Binding Protein
Mcm-Bp
Mcm-Binding Protein
Cellular Localisation Nucleus
Associates With Chromatin
Highly Associated With Chromatin In G1/S And S Phases
Reduced Binding To Chromatin In G2
And Further Decreased Binding In Early M Phase
It Then Reassociates With Chromatin In Late M Phase
Dissociates From Chromatin Later Than Component Of The Mcm Complex
Alternative Antibody Names Anti-Mini-Chromosome Maintenance Complex-Binding Protein antibody
Anti-Mcm-Bp antibody
Anti-Mcm-Binding Protein antibody
Anti-MCMBP antibody
Anti-C10orf119 antibody

Information sourced from Uniprot.org

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