• Immunoprecipitation analysis of 300 Mu g extracts of Raji cells using 3 Mu g MCM5 antibody (STJ115475). Western blot was performed from the immunoprecipitate using MCM5 antibody (STJ115475) at a dilution of 1:1000.
  • Immunofluorescence analysis of U-2 OS cells using MCM5 Rabbit polyclonal antibody (STJ115475) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MCM5 in paraffin-embedded human appendix using MCM5 Rabbit polyclonal antibody (STJ115475) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MCM5 in paraffin-embedded human lung cancer using MCM5 Rabbit polyclonal antibody (STJ115475) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using MCM5 Rabbit polyclonal antibody (STJ115475) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MCM5 antibody (1-230) (STJ115475)

SKU:
STJ115475

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MCM5 (1-230) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MCM5
Gene ID: 4174
Uniprot ID: MCM5_HUMAN
Immunogen Region: 1-230
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MCM5 (NP_006730.2).
Immunogen Sequence: MSGFDDPGIFYSDSFGGDAQ ADEGQARKSQLQRRFKEFLR QYRVGTDRTGFTFKYRDELK RHYNLGEYWIEVEMEDLASF DEDLADYLYKQPAEHLQLLE EAAKEVADEVTRPRPSGEEV LQDIQVMLKSDASPSSIRSL KSDMMSHLVKIPGIIIAASA VRAKATRISIQCRSCRNTLT NIAMRPGLEGYALPRKCNTD QAGRPKCPLDPYFIMPDKCK CVDFQTLKLQ
Function Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Protein Name Dna Replication Licensing Factor Mcm5
Cdc46 Homolog
P1-Cdc46
Database Links Reactome: R-HSA-176187
Reactome: R-HSA-176974
Reactome: R-HSA-68867
Reactome: R-HSA-68949
Reactome: R-HSA-68962
Reactome: R-HSA-69052
Cellular Localisation Nucleus
Chromosome
Associated With Chromatin Before The Formation Of Nuclei And Detaches From It As Dna Replication Progresses
Alternative Antibody Names Anti-Dna Replication Licensing Factor Mcm5 antibody
Anti-Cdc46 Homolog antibody
Anti-P1-Cdc46 antibody
Anti-MCM5 antibody
Anti-CDC46 antibody

Information sourced from Uniprot.org

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