| Host: | Rabbit |
| Applications: | WB/IHC-P/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Monoclonal |
| Clone ID : | S6MR |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000 IHC-P:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MCL1 |
| Gene ID: | 4170 |
| Uniprot ID: | MCL1_HUMAN |
| Immunogen Region: | 50-150 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human MCL1 (Q07820). |
| Immunogen Sequence: | GGEAGAVIGGSAGASPPSTL TPDSRRVARPPPIGAEVPDV TATPARLLFFAPTRRAAPLE EMEAPAADAIMSPEEELDGY EPEPLGKRPAVLPLLELVGE S |
| Post Translational Modifications | Cleaved by CASP3 during apoptosis. In intact cells cleavage occurs preferentially after Asp-127, yielding a pro-apoptotic 28 kDa C-terminal fragment. Rapidly degraded in the absence of phosphorylation on Thr-163 in the PEST region. Phosphorylated on Ser-159, by GSK3, in response to IL3/interleukin-3 withdrawal. Phosphorylation at Ser-159 induces ubiquitination and proteasomal degradation, abrogating the anti-apoptotic activity. Treatment with taxol or okadaic acid induces phosphorylation on additional sites. Ubiquitinated. Ubiquitination is induced by phosphorylation at Ser-159. Deubiquitinated by USP20.leading to increased stability. |
| Function | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis. |
| Protein Name | Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 Bcl-2-Like Protein 3 Bcl2-L-3 Bcl-2-Related Protein Eat/Mcl1 Mcl1/Eat |
| Database Links | Reactome: R-HSA-6785807 Reactome: R-HSA-9725370 |
| Cellular Localisation | Membrane Single-Pass Membrane Protein Cytoplasm Mitochondrion Nucleus Nucleoplasm Cytoplasmic Associated With Mitochondria |
| Alternative Antibody Names | Anti-Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 antibody Anti-Bcl-2-Like Protein 3 antibody Anti-Bcl2-L-3 antibody Anti-Bcl-2-Related Protein Eat/Mcl1 antibody Anti-Mcl1/Eat antibody Anti-MCL1 antibody Anti-BCL2L3 antibody |
Information sourced from Uniprot.org