| Host: | Rabbit |
| Applications: | IHC/IF/WB/ELISA |
| Reactivity: | Human/Rat/Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase MARCHF1 (Internal) is suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-2000 IHC 1:100-1:300 ELISA 1:40000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | MARCHF1 |
| Gene ID: | 55016 |
| Uniprot ID: | MARH1_HUMAN |
| Immunogen Region: | Internal |
| Specificity: | MARCH1 Polyclonal Antibody detects endogenous levels of 40603 protein. |
| Immunogen: | Synthesized peptide derived from the Internal region of human 40603. |
| Post Translational Modifications | Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins. |
| Function | E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system. |
| Protein Name | E3 Ubiquitin-Protein Ligase Marchf1 Membrane-Associated Ring Finger Protein 1 Membrane-Associated Ring-Ch Protein I March-I Ring Finger Protein 171 Ring-Type E3 Ubiquitin Transferase Marchf1 |
| Cellular Localisation | Golgi Apparatus Trans-Golgi Network Membrane Multi-Pass Membrane Protein Lysosome Membrane Cytoplasmic Vesicle Membrane Late Endosome Membrane Early Endosome Membrane Cell Membrane |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Marchf1 antibody Anti-Membrane-Associated Ring Finger Protein 1 antibody Anti-Membrane-Associated Ring-Ch Protein I antibody Anti-March-I antibody Anti-Ring Finger Protein 171 antibody Anti-Ring-Type E3 Ubiquitin Transferase Marchf1 antibody Anti-MARCHF1 antibody Anti-MARCH1 antibody Anti-RNF171 antibody |
Information sourced from Uniprot.org