• Western blot analysis of lysates from DU145 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-MARCHF1 antibody (Internal) (STJ94007)

SKU:
STJ94007

£48.00 - £252.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-E3 ubiquitin-protein ligase MARCHF1 (Internal) is suitable for use in Immunohistochemistry, Immunofluorescence, Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MARCHF1
Gene ID: 55016
Uniprot ID: MARH1_HUMAN
Immunogen Region: Internal
Specificity: MARCH1 Polyclonal Antibody detects endogenous levels of 40603 protein.
Immunogen: Synthesized peptide derived from the Internal region of human 40603.
Post Translational Modifications Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins.
Function E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.
Protein Name E3 Ubiquitin-Protein Ligase Marchf1
Membrane-Associated Ring Finger Protein 1
Membrane-Associated Ring-Ch Protein I
March-I
Ring Finger Protein 171
Ring-Type E3 Ubiquitin Transferase Marchf1
Cellular Localisation Golgi Apparatus
Trans-Golgi Network Membrane
Multi-Pass Membrane Protein
Lysosome Membrane
Cytoplasmic Vesicle Membrane
Late Endosome Membrane
Early Endosome Membrane
Cell Membrane
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Marchf1 antibody
Anti-Membrane-Associated Ring Finger Protein 1 antibody
Anti-Membrane-Associated Ring-Ch Protein I antibody
Anti-March-I antibody
Anti-Ring Finger Protein 171 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Marchf1 antibody
Anti-MARCHF1 antibody
Anti-MARCH1 antibody
Anti-RNF171 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance