Anti-MAPRE1 antibody (C-Term) [KT51] (STJA0005379)

SKU:
STJA0005379-100

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Host: Rat
Applications: WB/ICC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rat monoclonal antibody anti-EB1 (C-Term) is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: KT51
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: PBS + 0.05% NaN3
Purification: Antigen Affinity Purified
Dilution Range: WB 1:1000
IHC
ICC 1:200
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: Mapre1
Gene ID: 13589
Uniprot ID: MARE1_MOUSE
Immunogen Region: C-Term
Specificity: The antibody detects a 30 kDa* protein corresponding to the molecular mass of EB1 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB1 by immunocytochemistry in HeLa and A431 cells.
Immunogen: Clone KT51 was generated from a GST fusion protein containing amino acids from the C-terminal region of mouse EB1. This sequence is highly conserved in human and rat EB1.
Tissue Specificity Expressed within the midpiece of sperm tail (at protein level).
Post Translational Modifications Acetylation at Lys-220 by KAT2B/PCAF promotes dynamic kinetochore-microtubule interactions in early mitosis. Crotonylated by KAT5 during mitosis, promoting astral microtubule plasticity and dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation, thereby ensuring accurate spindle positioning in mitosis. Decrotonylated by HDAC3.
Function Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. Involved in mitotic spindle positioning by stabilizing microtubules and promoting dynamic connection between astral microtubules and the cortex during mitotic chromosome segregation. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. Acts as a regulator of autophagosome transport via interaction with CAMSAP2. Functions downstream of Rho GTPases and DIAPH1 in stable microtubule formation. May play a role in cell migration.
Protein Name Microtubule-Associated Protein Rp/Eb Family Member 1
Apc-Binding Protein Eb1
End-Binding Protein 1
Eb1
Database Links Reactome: R-MMU-141444
Reactome: -MMU-2467813
Reactome: -MMU-2500257
Reactome: -MMU-2565942
Reactome: -MMU-380259
Reactome: -MMU-380270
Reactome: -MMU-380284
Reactome: -MMU-380320
Reactome: -MMU-5620912
Reactome: -MMU-5663220
Reactome: -MMU-68877
Reactome: -MMU-8852276
Reactome: -MMU-8854518
Reactome: -MMU-9648025
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Spindle
Spindle Pole
Associated With The Microtubule Network At The Growing Distal Tip Of Microtubules
Also Enriched At The Centrosome
Alternative Antibody Names Anti-Microtubule-Associated Protein Rp/Eb Family Member 1 antibody
Anti-Apc-Binding Protein Eb1 antibody
Anti-End-Binding Protein 1 antibody
Anti-Eb1 antibody
Anti-Mapre1 antibody

Information sourced from Uniprot.org

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